Education
B.Sc., Bioengineering: Biotechnology Minor: Political Science
University of California, San Diego
Graduation Date: June, 2010
Experience
Senior Automation Engineer
Process Control Systems - AES, Oceanside, CA1/18-Present
- Expert knowledge of current state of the art control system development and integration
- Design batch recipes and implement recipe efficiency improvements for U/D bioprocesses
- Design OEM products; including control and hardware decisions for Bioreactor, Rocker, and ATF systems
- Contributed and developed lightweight DeltaV Object library for implementation as AES standard
- Develop custom GUI and windows services/program interfaces within DeltaV over VBA/C#/.NET
- Responsible for providing newly hired associates with controls and automation training, and technical onboarding
- Technical expert for developing integrated systems with all commonly used industrial protocols
Project Work
Shire, Glendale, CA2018
- Collaborated with Shire Eng. Staff to rewrite the B5 Buffer Preparation and Buffer Storage areas
- Developed phase and EM code to improve maintainability and robustness of legacy code base
- Rebuilt DeltaV MIMIC simulation system to allow for tiebacks and full simulation of facility IO
- Troubleshot and tested rewritten DeltaV code base to eliminate integration issues
- Created URS, FS, and SDS documents for all rewritten software objects
Automation Engineer
Process Control Systems - AES, Oceanside, CA4/15-12/17
- Expert knowledge of current state of the art biologics processes, and end-to-end plant design
- Design batch recipes and implement recipe efficiency improvements for U/D bioprocesses
- Implement DeviceNet, Profibus, Modbus, and Foundation Fieldbus based DCS networks
- Design and integrate automated SQL reporting services for stand-alone batch processes
- Develop custom GUI and windows services/program interfaces within DeltaV over VBA/C#/.NET
- Execute software, and recipe development within strict change control environments
- Technical expert for troubleshooting and modeling of GMP/GLP DeltaV PCS systems and ISA88
Project Work
Beohringer Ingelheim, Fremont, CA2015-2018
- Technical expert for troubleshooting in-process upstream and downstream DeltaV PCS systems
- Developed ‘one-source’ 24/7 resource for facility control systems; including all maintenance, metrology, and operational issues
- Analyzed in-line Profibus, and Foundation Fieldbus segment issues, providing detailed feedback signal reflections/noise, and estimated distance to issue
- Provided IT support for DeltaV Pro-Plus server restarts, communication issues, and maintenance
- Educated on-floor first line of defense workers about PCS and Bioprocess controls
- Developed control software for CIP, COP, SIP, TFF, Bioreactor, and Chromatography skids for new drug entrances to the CMO facility
- Lead failure analysis meetings, and presented the Eng. Dept. with solutions for improvement
- Created URS, FS, and SDS documents for all projects
- Collaborated with Boehringer Ingelheim Eng. staff to provide design solutions for facility fit
Gilead, Oceanside, CA2016
- Design and integrate a 2L bench scale bioreactor, with a Run Manager solution
- Develop import/export script for Data Management and bioprocess Run Time operations
- Design and integrate automated SQL reporting services for stand-alone batch processes
- Develop custom GUI and windows services/program interfaces within DeltaV over VBA/C#/.NET
BioMarin, Marin, CA2015
- Design and integrate automated SQL reporting services for stand-alone autoclave processes
- Develop custom GUI and windows services/program interfaces within DeltaV over VBA/C#/.NET
Engineer I - Early Stage Cell Culture
Research Materials Group - Genentech, So. San Francisco, CA8/14-3/15
- Solely responsible for developing a high-throughput 1mL cell culture system for generating research material for large scale screening experiments
- Implementing modern 40L lab-scale reactors and educating users about new equipment operation
- Optimization of cell culture processes and media formulations at 1mL to 40L scales
- Design and implementation of large scale bioprocess controls and robotic liquid-handling systems
- Design, conduct, and report analytical experiments detailing cell biology and protein biosynthesis
- Regularly present experimental results, process improvements, and technology advances
Associate Engineer - Early Stage Cell Culture
Research Materials Group - Genentech, So. San Francisco, CA3/13-7/14
- Management of various mammalian (CHO, HEK293) cell lines for transient material production
- Development of high-throughput micro-scale cell culture processes and screens
- Investigation and analysis of instrumentation, processes, and method improvements by DOE
- Collaborate with depts., and researchers to ensure logistics for generating research materials
Bioprocess Technician - Global Technical Operations
Cell Culture - Genentech, Oceanside, CA4/11-2/13
- Execution and troubleshooting of batch based cell culture operations in a strict cGMP environment
- Development of DeltaV code and software; including validation, qualification, and training modules
- Operate and audit commercial scale biologics equipment
Automation Skills
- Expert knowledge of DeltaV and PLC troubleshooting, code development and cGMP qualification
- DeltaV, C#, VB/VBA, SQL, Mimic, Finesse truBIO, EVOware, MATLAB, JMP, and DOE utilization
- Database integration and custom automated batch reporting
- Expert knowledge of TECAN EVO and BioMek FX liquid handling systems and scripting
Laboratory Skills
- Bioprocess experience with 1mL to 16,000L mammalian cell culture and harvest operations
- Optimizing PEI based transfections in various HEK293 and CHO cell lines
- Familiarity with laboratory techniques; PCR, DNA/RNA plasmid design, HPLC, western/eastern blots, electrophoresis/shift assays, cytometry, chromatography, TFF, single cell imaging
Publications
Bos AB, Duque JN, Bhakta S, Farahi F, Chirdon LA, Junutula JR, Harms PD, Wong AW. “Development of a semi-automated high throughput transient transfection system”. Journal of Biotechnology. 2014 June 20; 180:10-6
Bos AB, Luan P, Duque JN, Reilly D, Harms PD, Wong AW. “Optimization and automation of an end-to-end high throughput microscale transient protein production process.” Biotechnology and Bioengineering. 2015 Sep; 112(9):1832-42
Ohri R, Bhakta S, Fourie-O'Donohue A, Dela Cruz-Chuh J, Tsai SP, Cook R, Wei B, Ng C, Wong AW, Bos AB, Farahi F, Bhakta J, Pillow TH, Raab H, Vandlen R, Polakis P, Liu Y, Erickson H, Junutula JR, Kozak KR. “High-Throughput Cysteine Scanning To Identify Stable Antibody Conjugation Sites for Maleimide- and Disulfide-Based Linkers.” Bioconjugate Chemistry. 2018 Feb 21; 29(2):473-485
Posters and Presenstations
Bos AB, Duque JN, Wong AW. (2013). Automation of Small Scale Transient Cell Cultures.
(Poster Presentation) Genentech Protein Sciences Town Hall #2 2014, South San Francisco, CA.
(Poster Presentation) Genentech PTD Conference 2013, Asilomar, CA.
(Oral Presentation) Genentech PD Seminar 2014, South San Francisco, CA.
Bos AB, Luan P, Duque JN, Reilly D, Harms PD, Wong AW. (2015). Optimization of an End-to-End Microscale Transient Production Process.
(Poster Presentation) SLAS 2015 Poster Sessions, Washington D.C., MD